Immunofluorescent staining of 293T cells transiently transfected by pCMV6-ENTRY DCX (RC221891) using anti-DCX mouse monoclonal antibody (TA501778, green, upper left; merged, upper right). Cell nuclei were stained with DAPI (blue, lower left). 293T cells transfected with empty vector plasmid served as negative control ( merged, lower right) (1:100).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DCX (RC221891, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DCX. Positive lysates LY403598 (100ug) and LC403598 (20ug) can be purchased separately from OriGene.
Living HEK293T cells transfected with either RC221891 plasmid (red) or empty vector (blue) were immunostained by anti-DCX antibody (TA501778) or isotype control antibody (green), and then analyzed by flow cytometry (1:100).
Western blot analysis of extracts (35ug) from 2 tissues by using anti-DCX monoclonal antibody (1:250).