HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOK7 (RC219267, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOK7. Positive lysates LY406543 (100ug) and LC406543 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-DOK7 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DOK7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA504809)
Anti-DOK7 mouse monoclonal antibody (TA504809) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DOK7 (RC219267).
HEK293T cells transfected with either RC219267 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DOK7 antibody (TA504809), and then analyzed by flow cytometry.