Dicer siRNA Generation Kit

Easily generate functional siRNAs

The Dicer siRNA Generation Kit allows easy and cost-effective generation of a large number of small interfering RNAs (siRNAs) from full-length target genes. The kit mimics the natural RNA interference process by using recombinant human dicer enzyme to cleave in vitro transcribed dsRNA templates into a pool of 22bp siRNAs.

How does the Dicer siRNA Generation Kit work?

Applications

Citations

Recombinant Dicer efficiently converts large dsRNAs into siRNAs suitable for gene silencing
Myers, J.W., Jones, J.T., Meyer, T., & Ferrell, J.E. Jr; Nature Biotechnology. 2003, 21(3), 324-328

Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila melanogaster embryo lysate
Elbashir, S.M., Martinez, J., Patkaniowska, A., Lendeckel, W., & Tuschi, T; EMBO J. 2001, 20(23), 6877-88

Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems
Caplen, N.J., Parrish, S., Imani, F., Fire, A., & Morgan R.A; Proc Natl Acad Sci USA. 2001, 98(17), 9742-7

RNA interference
Sharp, P.A; Genes Dev. 2001, 15(5), 485-90