Dicer siRNA Generation Kit

Easily generate functional siRNAs

The Dicer siRNA Generation Kit allows easy and cost-effective generation of a large number of small interfering RNAs (siRNAs) from full-length target genes. The kit mimics the natural RNA interference process by using recombinant human dicer enzyme to cleave in vitro transcribed dsRNA templates into a pool of 22bp siRNAs.

How does the Dicer siRNA Generation Kit work?



Recombinant Dicer efficiently converts large dsRNAs into siRNAs suitable for gene silencing
Myers, J.W., Jones, J.T., Meyer, T., & Ferrell, J.E. Jr; Nature Biotechnology. 2003, 21(3), 324-328

Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila melanogaster embryo lysate
Elbashir, S.M., Martinez, J., Patkaniowska, A., Lendeckel, W., & Tuschi, T; EMBO J. 2001, 20(23), 6877-88

Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems
Caplen, N.J., Parrish, S., Imani, F., Fire, A., & Morgan R.A; Proc Natl Acad Sci USA. 2001, 98(17), 9742-7

RNA interference
Sharp, P.A; Genes Dev. 2001, 15(5), 485-90