Western Blot: DCLK3 Antibody - Samples: Lane 1, Marker: 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2, RT-4 Lane 3, U-251MG sp Lane 4, Human Plasma Lane 5, Liver Lane 6, Tonsil, Target weight: 74 Validation score: 2 Validation description: Supportive - Band of predicted size in kDa (+/-20%) with additional bands present.
Western Blot: DCLK3 Antibody - Western Blot was performed using affinity purified SEQer DCAMKL3 antibody, aa (138-237) antibody. The lanes contain 5-30ug of a whole cell extract. Final concentration of antibodies = 0.1ug/ml (1:10,000 dilution). The blot was probed overnight with the SEQer DCAMKL3 antibody, aa (138-237) antibody. Blot was then washed according to protocol and probed with goat-anti-Rabbit-HRP conjugate at 1:5000 dilution, washed and developed using chemiluminescence (film exposure 5-30sec). The protein was detected as represented by the band shown.
Immunohistochemical staining of human cerebellum shows cytoplasmic and nuclear positivity in Purkinje cells. Antibody dilution: 1:400. Image and statement courtesy of the Human Protein Atlas (HPA).