HEK293T cells transfected with either RC216274 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DAPK2 antibody (TA501087), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-DAPK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA501087)
Immunoprecipitation (IP) of DAPK2 by using TrueMab monoclonal anti-DAPK2 antibodies (Negative control: IP without adding anti-DAPK2 antibody.). For each experiment, 500ul of DDK tagged DAPK2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-DAPK2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.