HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYP2C9 (RC220997, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP2C9. Positive lysates LY400262 (100ug) and LC400262 (20ug) can be purchased separately from OriGene.
HEK293T cells transfected with either RC220997 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CYP2C9 antibody (TA503776), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CYP2C9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA503776)