HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYP2A6 (RC222995, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP2A
HEK293T cells transfected with either RC222995 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CYP2A6 antibody (TA503827), and then analyzed by flow cytometry.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CYP2A6 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CYP2A6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA503827)
Anti-CYP2A6 mouse monoclonal antibody (TA503827) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CYP2A6 (RC222995).