Stable Cell Lines Request Form

Name of host cell line*

Type*

Can you provide the host cell line?*

Yes
No (please state where it is commericially available from below)

Please note that the construction of a stable cell line in our 293HEK Cells (Human Embryonic Kidney) might be a cheaper option.

Company name and Cat#

Cell line required*

Stable cell line over-expressing a specific cDNA
Knockdown stable cell line

Gene accession number (mRNA NCBI reference)

Can you provide the DNA gene template (along with its coding sequence) for sub-cloning?

Preferred promoter

suCMV
EF1a
Tetracycline inducible suCMV
Other

Please specify promoter

Do you need a tag (6His-tag, HA, Myc, V5, GFP, RFP, CFP...)?

No, do not include a tag
Put a tag at N-terminal of the gene
Put a tag at C-terminal of the gene

Please specify the tag you prefer

Preferred marker

Expression validation in Western Blot

Details of antibody

Gene accession number (mRNA NCBI reference)

Can you provide the knockdown sequence?

Constitutive or inducible expression of the shRNA?

Preferred marker

Additional comments or requirements (mutagenesis, negative control cell line, bicistronic expression etc.)

Name*

Telephone Number*

Email Address*

Organization :

Address*

Street Address

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