Custom Baculovirus Protein Expression
Fast and reliable protein expression from insect cells
AMSBIO offers a custom baculovirus protein expression service, which utilizes a high-level expression system for the fast production of high quality recombinant proteins. The baculovirus-insect cell protein expression system is a widely used system for the production of recombinant proteins, which have complete biological function and post-translational modifications. This flexible system is safe, reliable and scalable, and can accommodate large insert proteins, making it an attractive option for use in many research areas.
Our Services
AMSBIO's baculovirus protein expression service consists of two distinct phases:
Phase 1
- Amplification/isolation of the gene of interest out of a customer-supplied vector and subcloning it into a transfer vector with His-tag
- Sequence confirmation of insert
- Transformation of E. coli with baculoviral DNA and gene of interest
- Culture of E. coli and purification using mini-prep to produce bacmid (recombinant viral DNA)
- Transfection of Sf9 insect cells with bacmid
- Plaque purification and viral titer assay
- Small-scale culture to test expression level
Phase 2
- Virus stock preparation
- Large-scale culture and infection of insect cells for protein expression
- Purification from 1 liter of infected insect cells using affinity column of Ni-NTA or other column
Products
Following completion of the service you will receive the following:
- 5µg transfer vector
- Bacmid
- Recombinant virus
- Protein
- COA
Schematic Representation
Protein Expression Request Form
Let's get started
Complete our service request form and we will contact you to discuss your requirements.