Recombinant Monoclonal Antibody Service
Rapid and efficient monoclonal antibody development
Our high quality recombinant monoclonal antibody development services offer an superior alternative to hybridoma fusion platforms, as we directly isolate IgG genes from B cells of immunized animals. This enables us to produce the antibodies you need in as little as 4-5 months. With both mouse and rabbit monoclonal antibody development available, we can tailor our service to directly suit your research needs. We also provide thousands of high quality off-the-shelf monoclonal and polyclonal antibodies, browse our collection here.
Benefits
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- Short development process (4-5 months)
- High project success rate (80-90%)
- High cloning efficiency – thousands of clones from one immunized animal
- Direct acquisition of antibody IgG gene sequence, easy to maintain and transmit information
- High quality consistency between batches, and possible to automate the process
Applications
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- Therapeutic antibody drug development
- Immunohistochemistry
- Companion diagnostics
- Immunoblotting
- Flow cytometry
Our Services
Comparison between rabbit and mouse monoclonal antibodies
| Feature | Rabbit Monoclonal Antibodies | Mouse Monoclonal Antibodies |
|---|---|---|
| Affinity | +++++ | ++ |
| New IHC clones | +++++ | +++ |
| Old and common IHC clones | +++ | ++++ |
| Maturity for antibody drug development | ++ | +++++ |
| Common IVD clones | ++ | ++++ |
Rabbit Monoclonal Antibody Development
Rabbit monoclonal antibodies are increasing in popularity for use in immunological assay development, particularly in immunohistochemistry (IHC) applications. Currently, only 5 FDA approved companion diagnostic IHC antibodies are rabbit monoclonal antibodies. However, this new proprietary technology platform offers opportunity for a great technology breakthrough in this area, with shortened development time and acquisition of IgG gene sequence without the loss of hybridoma cells.
Service Details and Deliverables
| Step | Service Details | Deliverables | Estimated Production Cycle |
|---|---|---|---|
| Immunogen preparation | Plasmid construction (mammalian cell produced immunogens or supplied by customer) | 7-10 weeks | |
| Animal immunization | Immunize two rabbits against the same antigen | Crude sera and ELISA data on anti-serum titer test | 5-8 weeks |
| B cell isolation and amplification | PBMC collection and cryopreservation
Target positive B cell enrichment and single B cell expansion | 100 µl B cell supernatant
B cell supernatant ELISA test data | 2-3 weeks |
| cDNA cloning, sequencing and storage | IgG gene cloning
Expression vector construction Small-scale antibody production and validation Select 1-2 positive clones for full-length IgG sequencing and sequencing analysis | Sequencing report of one clone
ELISA data | 3-4 weeks |
| Antibody production and validation | Antibody production, purification and QC validation | 1 mg purified recombinant antibody | 3-4 weeks |
Mouse Monoclonal Antibody Development
Mouse is often the first choice when it comes to a therapeutic antibody drug developmental programs. Despite this, mouse monoclonal development comes with many limitations, such as low fusion efficiency of hybridoma and unstable hybridoma cells. Our mouse monoclonal antibody development service overcomes these issues by directly isolating IgG genes from B cells, letting you receive the antibodies you need in record time.
Service Details and Deliverables
| Step | Service Details | Deliverables | Estimated Production Cycle |
|---|---|---|---|
| Immunogen preparation | Plasmid construction (mammalian cell produced immunogens or supplied by customer) | 7-10 weeks | |
| Animal immunization | Immunize two mice against the same antigen | Crude sera and ELISA data on anti-serum titer test | 8-12 weeks |
| Collect mouse splenocytes | Dissect animal and cryopreservation
Target positive B cell enrichment and single B cell expansion | 100 µl B cell supernatant
B cell supernatant ELISA test data | 4 weeks |
| cDNA cloning, sequencing and storage | IgG gene cloning
Expression vector construction Small-scale antibody production and validation Select 1-2 positive clones for full-length IgG sequencing and sequence analysis | Sequencing report of one clone
ELISA data | 2 weeks |
| Antibody production and validation | Antibody production and identification
Antibody mass production and purification Antibody function identification | Antibody production
Purification and QC validation | 3-4 weeks |
Antibody Development Request Form
Let's get started
Complete our service request form and we will contact you to discuss your requirements.