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Lentivirus & Adenovirus & AAV Request Form
Service Required
*
Select Required Service
Lentivirus ORF Expression
Lentivirus shRNA Expression
AAV
Lentivirus microRNA
Lentivirus anti miRNA
Adenovirus
Integrase-Deficient Lentivirus
Lentiviruses for SARS-CoV-2 Research
Gene accession number (mRNA NCBI reference)
Can you provide the DNA gene template (along with its coding sequence) for sub-cloning?
Select one
Yes
No, I need AMSBIO to either synthesize or obtain it from a cDNA collection
Preferred promoter
Select promoter
CMV
H1
U6
Other (please specify below)
Promoter name
Tag
Select tag
Flag-His
GFP
RFP
Serotype
Select serotype
AAV1
AAV2
AAV5
AAV6
AAV7
AAV8
AAV9
Gene/section to be cloned
Select one
cDNA
shRNA
Packaging required
Select one
Small scale (500uL of rAAV at 1x1010-11vp/mL)
Large scale (400uL of rAAV at 1x1013vp/mL)
Not required
Gene accession number (mRNA NCBI reference)
Can you provide the knockdown sequence?
Select one
Yes
No, I need AMSBIO to design it
Please select the promoter you would like to use
Select one
U6
Optional inducible H1*
* Our optional tetracycline inducible promoter can be used for high constitutive expression without any induction. The inducible expression is just an optional feature when a repressor is present in advance.
Preferred marker
Select marker
Blasticidin
Puromycin
GFP-Blasticidin
RFP-Blasticidin
GFP-Puromycin
RFP-Puromycin
Required quantity of virus (titer should be around 1x10^(6-7) IFU/mL
Select one
0.5ml (by default)
Other (please specify below)
Quantity required (mL)
Gene accession number (mRNA NCBI reference)
What would you like to express?
Select one
cDNA/ORF
shRNA
miRNA
Which promoter would you like to use?
Do you need a tag?
Volume and titer of adenovirus required
Gene accession number (mRNA NCBI reference)
Can you provide the DNA gene template (along with its coding sequence) for subcloning?
Choose one
Yes
No, I need AMSBIO to either synthesize it or obtain it from a cDNA collection
Preferred promoter
Select one
CAG
suCMV
EF1a
Optional inducible suCMV*
Other (please specify below)
* Our optional tetracycline inducible promoter can be used for high constitutive expression without any induction. The inducible expression is just an optional feature when a repressor is present in advance.
Promoter details
Do you need a tag?
Choose one
No, do not include a tag
Yes, put a tag at N-terminal of the gene (please specify below)
Yes, put a tag at C-terminal of the gene (please specify below)
Required tag
Required marker
Select marker
Blasticidin
Neomycin
Puromycin
GFP-Blasticidin
RFP-Blasticidin
GFP-Puromycin
RFP-Puromycin
Firefly luciferase
Required quantity (titer should be around 1x10^(6-7) IFU/mL
Select one
0.5 mL (by default)
Other (please specify below)
Quantity (mL)
Additional information
Name
*
Telephone Number
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Email Address
*
Organization :
Address
*
Street Address
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