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Lentivirus & Adenovirus & AAV Request Form
Service Required :
*
Select Required Service
Lentivirus ORF Expression
Lentivirus shRNA Expression
AAV
Lentivirus microRNA
Lentivirus anti miRNA
Adenovirus
Gene accession number (mRNA NCBI reference)
Can you provide the DNA gene template (along with its coding sequence) for sub-cloning?
Select one
Yes
No, I need AMSBIO to either synthesize or obtain it from a cDNA collection
Preferred promoter
Select promoter
CMV
H1
U6
Other (please specify below)
Promoter name
Tag
Select tag
Flag-His
GFP
RFP
Serotype
Select serotype
AAV1
AAV2
AAV5
AAV6
AAV7
AAV8
AAV9
Gene/section to be cloned
Select one
cDNA
shRNA
Packaging required
Select one
Small scale (500uL of rAAV at 1x1010-11vp/mL)
Large scale (400uL of rAAV at 1x1013vp/mL)
Not required
Gene accession number (mRNA NCBI reference)
Can you provide the knockdown sequence?
Select one
Yes
No, I need AMSBIO to design it
Please select the promoter you would like to use
Select one
U6
Optional inducible H1*
* Our optional tetracycline inducible promoter can be used for high constitutive expression without any induction. The inducible expression is just an optional feature when a repressor is present in advance.
Preferred marker
Select marker
Blasticidin
Puromycin
GFP-Blasticidin
RFP-Blasticidin
GFP-Puromycin
RFP-Puromycin
Required quantity of virus (titer should be around 1x10^(6-7) IFU/mL
Select one
0.5ml (by default)
Other (please specify below)
Quantity required (mL)
Gene accession number (mRNA NCBI reference)
What would you like to express?
Select one
cDNA/ORF
shRNA
miRNA
Which promoter would you like to use?
Do you need a tag?
Volume and titer of adenovirus required
Gene accession number (mRNA NCBI reference)
Can you provide the DNA gene template (along with its coding sequence) for subcloning?
Choose one
Yes
No, I need AMSBIO to either synthesize it or obtain it from a cDNA collection
Preferred promoter
Select one
CAG
suCMV
EF1a
Optional inducible suCMV*
Other (please specify below)
* Our optional tetracycline inducible promoter can be used for high constitutive expression without any induction. The inducible expression is just an optional feature when a repressor is present in advance.
Promoter details
Do you need a tag?
Choose one
No, do not include a tag
Yes, put a tag at N-terminal of the gene (please specify below)
Yes, put a tag at C-terminal of the gene (please specify below)
Required tag
Required marker
Select marker
Blasticidin
Neomycin
Puromycin
GFP-Blasticidin
RFP-Blasticidin
GFP-Puromycin
RFP-Puromycin
Firefly luciferase
Required quantity (titer should be around 1x10^(6-7) IFU/mL
Select one
0.5 mL (by default)
Other (please specify below)
Quantity (mL)
Additional information
Name
*
Telephone Number
*
Email Address
*
Organization :
Address
*
Street Address
*
*
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