Vaccinia Virus (VACV)
Recombinant VACV has several advantages including large carrying capacity (25-30 kb), wide tropism, and ability to replicate without host machinery. It has recently shown great promise in a broad range of clinical applications, including vaccine development and oncolytic therapy.
Our Services
Types of VACV services offered
AMSBIO offers both cloning and packaging services of VACV vectors, suitable for either in vitro or in vivo studies.
- VACV vector cloning in BACYAC backbone for both attenuated Western Reserve (WR) and Modified Vaccinia virus Ankara (MVA) strains
- VACV virus packaging
VACV production and quality control (QC)
For virus packaging (Figure 1), a noninfectious BACYAC plasmid carrying the gene of interest (GOI) is transfected into packaging cells and infected with a helper non-replicating fowlpox virus. Because VACV is capable of infecting a broad range of host cells, multiple cell lines can be used to package the recombinant virus. At AMSBIO different cell lines are used for VACV rescuing and amplification in preparation of virus stocks. Following VACV rescuing and amplification steps, plaque purification is used to establish a clonal virus stock which is then further amplified. Both extracellular enveloped virus (EEV) and intracellular mature virus (IMV) are harvested from the supernatant and cell lysate, respectively. EEV is PEG concentrated and combined with IMV. For ultra-purified VACV, viral particles are further purified by sucrose gradient purification.
Lentivirus & AAV & more Request Form
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