MSCV Retrovirus Packaging
High titer and consistent MSCV vectors to overcome restricted expression in ES and EC cells
Recombinant murine stem cell virus (MSCV) vectors serve as efficient viral vector tools for achieving robust expression of genes of interest in embryonic stem (ES) cells, embryonal carcinoma (EC) cells and hematopoietic stem (HS) cells, along with several other mammalian cell lines. In this retroviral vector system, the presence of a strategically designed 5’LTR derived from the murine stem cell PCMV virus in the MSCV retroviral vector contributes to the transcriptional activation of target genes in pluripotent cell lines. This helps to overcome the restricted expression of target genes in ES and EC cells, typically observed with MMLV retroviral vectors.
Our Services
Types of MSCV retrovirus offered
AMSBIO offers a series of proprietary technologies and reagents that have greatly improved recombinant MSCV production protocols in terms of titer, purity, viability and consistency, especially for the MSCV vector systems used in our vector cloning services.
- VSV-G pseudotyped MSCV retrovirus
MSCV retrovirus production and quality control (QC)
For MSCV retrovirus manufacturing (Figure 1), the transfer vector carrying the gene of interest (GOI) is co-transfected with our proprietary envelop vector encoding VSV-G and packaging vector encoding Gag and Pol. After a short incubation period, the supernatant is collected and centrifuged to remove cell debris and then filtered. Viral particles are subsequently concentrated with PEG. For ultra-purified MSCV retrovirus (in vivo grade), viral particles are further purified and concentrated by sucrose cushion centrifugation. We use a qPCR-based approach to measure the functional titer of MSCV.
Lentivirus & AAV & more Request Form
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