MMLV Retrovirus Packaging
High titer and consistent MMLV vectors
Recombinant Moloney murine leukemia virus (MMLV) retroviral vectors serve as efficient viral vector tools for introducing genes permanently into a wide variety of dividing cells. This retroviral system came into prominence as a gene delivery method for generating iPS cells. AMSBIO utilises a series of proprietary technologies and reagents that have greatly improved recombinant MMLV retrovirus production protocols in terms of titer, purity, viability and consistency, especially for the MMLV vector systems used in our vector cloning services. Read below to find out more.
Our Services
Types of MMLV retrovirus offered
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- VSV-G pseudotyped wildtype MMLV retrovirus
- VSV-G pseudotyped self-inactivating MMLV retrovirus
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MMLV retrovirus production and quality control (QC)
For MMLV retrovirus manufacturing (Figure 1), the transfer vector carrying the gene of interest (GOI) is co-transfected into packaging cells with our proprietary envelope vector encoding VSV-G and our packaging vector encoding Gag and Pol. After a short incubation period, the supernatant is collected and centrifuged to remove cell debris and then filtered. Viral particles are subsequently concentrated with PEG. For ultra-purified MMLV retrovirus (in vivo grade), viral particles are further purified and concentrated by sucrose cushion centrifugation. We use a qPCR-based approach to measure the functional titer of MMLV.
Lentivirus & AAV & more Request Form
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