HSV Cloning and Packaging
Recombinant herpes simplex virus (HSV) has recently emerged as a promising viral vector in gene therapy and cancer therapy, with a variety of applications including gene delivery into the nervous system, as oncolytic agents, and for the development of vaccines against cancer, HSV and other infectious diseases. HSV vectors offer several advantages such as natural neurotropism, the ability to persist in a latent, episomal state throughout the lifetime of host cells, and their large cargo carrying capacity. These features make them the ideal viral vehicles for a range of in vitro and in vivo gene delivery needs.
Our Services
Types of HSV services offered
AMSBIO offers design and construction of HSV vectors that can be used to reconstitute live viruses, including wildtype and attenuated viruses as well as replication-defective amplicons. We also provide packaging services for both research-grade and GMP-grade HSV.
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- HSV vector cloning in BAC or BACYAC backbone
- HSV-1 virus packaging
- HSV-1 amplicon vector cloning
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HSV production and quality control (QC)
For HSV packaging (Figure 1), the BACYAC plasmid carrying the gene of interest (GOI) is transfected along with Cre plasmid into packaging cells. Nonfluorescent plaques are then selected to purify virus from which the BACYAC backbone is removed. Then, selected plaques are applied to new dishes to amplify HSV. For ultra-purified HSV, viral particles are further purified by sucrose density gradient centrifugation and concentrated.
Lentivirus & AAV & More Request Form
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