Applications of Collagenase NB and Neutral Protease NB
Find the best tissue dissociation enzymes for your research
To ensure consistent results, you should endeavour to follow the cell isolation protocol best suited to your application. The tissue/cell type you are using alters the required Collagenase NB concentration, as well as the isolation procedure. Suggested concentrations should be considered as starting points and the progress of the digestion process should be monitored visually in order to determine the optimal conditions.
Choose your intended tissue/cell type below to discover which Collagenase NB products are best suited to your application and download the protocols.
By Cell Type
Isolation of Acinar Cells with Collagenase NB
We recommend using Collagenase NB 8 Broad Range for isolation of acinar cells from human pancreatic tissue. Collagenase NB 8 Broad Range is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzyme | Conditions | Protocol |
|---|---|---|---|
| Human | Collagenase NB 8 | 0.05-0.1 PZ U/ml, | Download Protocol |
| 1 h, 37 °C |
Isolation of Adipocytes/Adipose-Derived Stem Cells with Collagenase NB
We recommend using Collagenase NB 4 Standard Grade for isolation of adipocytes/adipose-derived stem cells (ADSCs) from human and mouse tissue. Collagenase NB 4 Standard Grade has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.
For clinical applications, we recommend either using Collagenase NB 6 GMP Grade or Collagenase AF-1 with Neutral Protease AF. Collagenase NB 6 has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Research Application | Enzyme | Conditions | Protocol |
|---|---|---|---|---|
| Human | Basic | Collagenase NB 4 | 0.2-0.3 PZ U/ml*, | Download Protocol |
| 30-60 min, 37 °C | ||||
| Murine | Basic | Collagenase NB 4 | 0.2-0.3 PZ U/ml**, | Download Protocol |
| 30-60 min, 37 °C | ||||
| Human | Clinical | Collagenase NB 6 | 0.2-0.3 PZ U/ml*, | Download Protocol |
| 30-60 min, 37 °C | ||||
| Human | Clinical | Collagenase AF-1 | 0.4 PZ U/ml++ & 0.2 DMC U/ml++, | Download Protocol |
| Neutral Protease AF | 50 min, 37 °C |
Isolation of Amniotic Cells from Human Fetal Tissue with Collagenase NB
We recommend using Collagenase NB 8 Broad Range for isolation of amnion cells from human fetal tissue. Collagenase NB 8 Broad Range is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzyme | Conditions | Protocol |
|---|---|---|---|
| Human | Collagenase NB 8 | 3.5-5.5 PZ U/ml, | Download Protocol |
| 3-5 h, 37 °C |
Isolation of Cardiomyocytes from Cardiac Muscle Tissue
We recommend using Collagenase NB 8 Broad Range for isolation of cardiomyocytes from human and rat cardiac muscle tissue. Collagenase NB 8 Broad Range is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzyme | Conditions | Protocol |
|---|---|---|---|
| Rat (adult) | Collagenase NB 8 | 0.5 PZ U/ml, | Download Protocol |
| 45-60 min, 37 °C | |||
| Human | Collagenase NB 8 | 0.8-1.0 PZ U/ml, | Download Protocol |
| 2-3.5 h, 37 °C |
Isolation of Chondrocytes with Collagenase NB
We recommend using Collagenase NB 4 Standard Grade for isolation of chondrocytes from murine, bovine and human cartilage. Collagenase NB 4 Standard Grade has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.
For clinical applications, we recommend using Collagenase NB 6 GMP Grade has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Research Application | Enzyme | Conditions | Protocol |
|---|---|---|---|---|
| Bovine | Basic | Collagenase NB 4 | 0.2-0.3 PZ U/ml, | Download Protocol |
| 14-16 h, 37 °C | ||||
| Human | Basic | Collagenase NB 4 | 0.3-0.4 PZ U/ml, | Download Protocol |
| 4-16 h, 37 °C | ||||
| Mouse (newborn) | Basic | Neutral Protease NB | Consecutive digestion steps: | Download Protocol |
| 0.5-1 DMC U/ml (NP), | ||||
| 15-20 min, 37 °C | ||||
| Collagenase NB 4 | 0.3 PZ U/ml (NB 4), | |||
| 30-45 min, 37 °C | ||||
| Human | Clinical | Collagenase NB 6 | 0.3-0.4 PZ U/ml, | Download Protocol |
| 4-16 h, 37 °C |
Isolation of Chorion Cells from Human Fetal Tissue with Collagenase NB
We recommend using Collagenase NB 8 Broad Range for isolation of chorion cells from human fetal tissue. Collagenase NB 8 Broad Range is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzyme | Conditions | Protocol |
|---|---|---|---|
| Human | Collagenase NB 8 | 3.5-5.5 PZ U/ml, | Download Protocol |
| 3-5 h, 37 °C |
Isolation of Endometrial Cells from Human Cervical Tissue with Collagenase NB
We recommend using Collagenase NB 4 Standard Grade or Collagenase NB 6 GMP Grade for isolation of endometrial cells from human cervical tissue. Collagenase NB 4 Standard Grade has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. Collagenase NB 6 GMP Grade has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzymes | Conditions | Protocol |
|---|---|---|---|
| Human | Collagenase NB 4 | 0.2-0.3 PZ U/ml, | Download Protocol |
| or | 30 min, 37 °C | ||
| Collagenase NB 6 |
Isolation of Endothelial Cells from Aorta Tissue
We recommend using Collagenase NB 4 Standard Grade for isolation of endothelial cells from rodent aortas. Collagenase NB 4 Standard Grade has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzymes | Conditions | Protocol |
|---|---|---|---|
| Rodent | Collagenase NB 4 | 0.12-0.15 PZ U/ml, | Download Protocol |
| 10-20 min, 37 °C |
Isolation of Endothelial Cells From Human Foreskin and Umbilical Vein with Collagenase NB
We recommend using Collagenase NB 4 Standard Grade or Collagenase NB 6 GMP Grade for isolation of endothelial cells from human foreskin or umbilical vein. Collagenase NB 4 Standard Grade has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. Collagenase NB 6 GMP Grade has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzymes | Conditions | Protocol |
|---|---|---|---|
| Human (HDMEC) | Collagenase NB 4 | 0.5-0.7 PZ U/ml, | Download Protocol |
| or | 0.5-2 h, 37 °C | ||
| Collagenase NB 6 | |||
| Human (HUVEC) | Collagenase NB 4 | 0.15-0.25 PZ U/ml, | Download Protocol |
| or | 0.5-2 h, 37 °C | ||
| Collagenase NB 6 |
Isolation of Epithelial Cells from Gastric Mucosa with Collagenase NB
We recommend using Collagenase NB 4G Proved Grade for isolation of epithelial cells from gastric mucosa. Collagenase NB 4G Proved Grade has a collagenase activity of ≥ 0.18 PZ U/mg and a balanced mix of proteolytic side activities.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzymes | Conditions | Protocol |
|---|---|---|---|
| Human | Collagenase NB 4G | 0.25-0.3 PZ U/ml, | Download Protocol |
| RT |
Isolation of Epithelial Cells from Murine Mammary Glands with Collagenase NB
We recommend using Collagenase NB 4 Standard Grade for isolation of epithelial cells from murine mammary gland. Collagenase NB 4 Standard Grade has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzymes | Conditions | Protocol |
|---|---|---|---|
| Mouse | Collagenase NB 4 | 0.34 PZ U/ml, | Download Protocol |
| 15-25 min, 37 °C |
Isolation of Epithelial Cells from Thyroid Glands with Collagenase NB
We recommend using Collagenase NB 4 Standard Grade or Collagenase NB 6 GMP Grade for isolation of epithelial cells from human thyroid glands. Collagenase NB 4 Standard Grade has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. Collagenase NB 6 GMP Grade has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzymes | Conditions | Protocol |
|---|---|---|---|
| Human | Collagenase NB 4 | 0.25-0.3 PZ U/ml, | Download Protocol |
| or | RT | ||
| Collagenase NB 6 |
Isolation of Fibroblasts from Skin with Collagenase NB
We recommend using Collagenase NB 4G for isolation of fibroblasts from human and rodent skin. Collagenase NB 4G Proved Grade has a collagenase activity of ≥ 0.18 PZ U/mg and a balanced mix of proteolytic side activities.
For clinical applications, we recommend using Collagenase NB 6 GMP Grade. Collagenase NB 6 GMP Grade has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Research Application | Enzymes | Conditions | Protocol |
|---|---|---|---|---|
| Human | Basic | Collagenase NB 4G | 0.26 PZ U/ml, | Download Protocol |
| 2 h, 37 °C | ||||
| Rodent | Basic | Collagenase NB 4G | 0.2-0.5 PZ U/ml, | Download Protocol |
| 12-20 h, 37 °C | ||||
| Human | Clinical | Collagenase NB 6 | 0.2-0.3 PZ U/ml, | Download Protocol |
| 2 h, 37 °C |
Isolation of Hepatocytes from Liver Tissue with Collagenase NB
We recommend using Collagenase NB 4G Proved Grade for isolation of hepatocytes from human, mouse and rat liver tissue. Collagenase NB 4G Proved Grade has a collagenase activity of ≥ 0.18 PZ U/mg and a balanced mix of proteolytic side activities.
For pig liver tissue, we recommend using Collagenase NB 8 Broad Range. Collagenase NB 8 Broad Range characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.
For clinical applications, we recommend using Collagenase NB 6 GMP Grade. Collagenase NB 6 GMP Grade has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Research Application | Enzymes | Conditions | Protocol |
|---|---|---|---|---|
| Human | Basic | Collagenase NB 4G | 0.25-0.35 PZ U/ml, | Download Protocol |
| 10-20 min, 37 °C | ||||
| Mouse | Basic | Collagenase NB 4G | 0.08-0.11 PZ U/ml, | Download Protocol |
| 5-15min, 37 °C | ||||
| Pig | Basic | Collagenase NB 8 | 0.2-0.3 PZ U/ml | Download Protocol |
| Rat (adult) | Basic | Collagenase NB 4G | 0.12-0.15 PZ U/ml, | Download Protocol |
| 10-25 min, 37 °C | ||||
| Human | Clinical | Collagenase NB 6 | 0.25-0.35 PZ U/ml, | Download Protocol |
| 10-20 min, 37 °C |
Isolation of Islets of Langerhans from Pancreatic Tissue with Collagenase NB
| Species | Research Application | Enzymes | Conditions | Protocol |
|---|---|---|---|---|
| Human | Basic | Collagenase NB 1 Premium Grade | Per gram tissue: | |
| 20 PZ U (NB 1), | ||||
| Neutral Protease NB | 0.8-1.5 DMC U (NP), | |||
| 15-30 min, 37 °C | ||||
| Porcine (adult) | Basic | Collagenase NB 8 | Per gram tissue: | Download Protocol |
| 4.4 PZ U (NB 8), | ||||
| Neutral Protease NB | 0.5-0.7 DMC U (NP), | |||
| 37 °C | ||||
| Porcine (neonatal) | Basic | Collagenase NB 4 | 0.17-0.2 PZ U/ml, | Download Protocol |
| 10-20 min, 37 °C | ||||
| Rodent | Basic | Collagenase NB 8 | 1.5-1.7 PZ U/ml | Download Protocol |
| 14-17 min, 37 °C | ||||
| Human | Clinical | Collagenase NB 1 GMP Grade | Per gram tissue: | |
| 20 PZ U (NB 1), | ||||
| Neutral Protease NB GMP Grade | 0.8-1.5 DMC U (NP), | |||
| 15-30 min, 37 °C | ||||
| Porcine (adult) | Clinical | Collagenase NB 1 | Per gram tissue: | |
| 15-18 PZ U (NB 1), | ||||
| Neutral Protease NB GMP Grade | 0.8-1.3 DMC U (NP), | |||
| 15-30 min, 37 °C | ||||
| Porcine (neonatal) | Clinical | Collagenase NB 6 | 0.17-0.2 PZ U/ml, | |
| 10-20 min, 37 °C |
Isolation of Leukocytes from Murine Skin with Collagenase NB
We recommend using Collagenase NB 7D Gentle Grade for isolation of leukocytes from murine skin. Collagenase NB 7D Gentle Grade is characterized by slightly higher collagenase activity with low tryptic activities ideal to preserve cell-surface proteins.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzyme | Conditions |
|---|---|---|
| Mouse | Collagenase NB 7D | 0.18-0.22 PZ U/ml, |
| 30 min, 37 °C |
Isolation of Lung Cells with Collagenase NB
We recommend using Collagenase NB 8 Broad Range for isolation of lung cells from human tissue. Collagenase NB 8 Broad Range characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.
For murine lung tissue, we recommend using Collagenase NB 4 Standard Grade. Collagenase NB 4 Standard Grade has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzyme | Conditions | Protocol |
|---|---|---|---|
| Mouse | Collagenase NB 4 | 0.2 PZ U/ml, | Download Protocol |
| 1 h, 37 °C | |||
| Human | Collagenase NB 8 | 3.5-5.5 PZ U/ml, | |
| 3-5 h, 37 °C |
Isolation of Mastocytes from Intestinal Mucosa with Collagenase NB
We recommend using Collagenase NB 4G Proved Grade for mastocytes from human intestinal mucosa. Collagenase NB 4G Proved Grade has a collagenase activity of ≥ 0.18 PZ U/mg and a balanced mix of proteolytic side activities.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzyme | Conditions | Protocol |
|---|---|---|---|
| Human | Collagenase NB 4G | 0.5 PZ U/ml, | Download Protocol |
| 1 h, 37 °C |
Isolation of Melanoma Cells from Murine Skin with Collagenase NB
We recommend using Collagenase NB 8 Broad Range for isolation of melanoma cells from murine skin. Collagenase NB 8 Broad Range is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzyme | Conditions |
|---|---|---|
| Mouse | Collagenase NB 8 | 0.12 PZ U/ml, |
| 30 min, 37 °C |
Isolation of Myocytes from Skeletal Muscle Tissue with Collagenase NB
We recommend using Collagenase NB 8 Broad Range for isolation of myocytes from human skeletal muscle. Collagenase NB 8 Broad Range characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzyme | Conditions | Protocol |
|---|---|---|---|
| Human | Collagenase NB 8 | 0.4-0.6 PZ U/ml, | Download Protocol |
| 37 °C |
Isolation of Neurons from Dorsal Root Ganglia with Collagenase NB
We recommend using Collagenase NB 4 Standard Grade or Collagenase NB 6 GMP Grade for isolation of neurons from human dorsal root ganglia. Collagenase NB 4 Standard Grade has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. Collagenase NB 6 GMP Grade has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.
For rodent dorsal root ganglia, we recommend using Collagenase NB 4 Standard Grade.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzymes | Conditions | Protocol |
|---|---|---|---|
| Human | Collagenase NB 4 | 0.15-0.25 PZ U/ml, | Download Protocol |
| or | 30-60 min, 37 °C | ||
| Collagenase NB 6 | |||
| Rodent | Collagenase NB 4 | 0.15-0.25 PZ U/ml, | Download Protocol |
| 30-60 h, 37 °C** |
Isolation of Oocytes from Xenopus laevis with Collagenase NB
We recommend using Collagenase NB 4G for isolation of oocytes from Xenopus laevis. Collagenase NB 4G Proved Grade has a collagenase activity of ≥ 0.18 PZ U/mg and a balanced mix of proteolytic side activities.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzyme | Conditions | Protocol |
|---|---|---|---|
| Frog | Collagenase NB 4G | 0.3-0.5 PZ U/ml, | Download Protocol |
| 1.5-2 h, RT |
Isolation of Osteoblasts with Collagenase NB
We recommend using Collagenase NB 8 Broad Range with Neutral Protease NB for isolation of osteoblasts from human tissue. Collagenase NB 8 Broad Range is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities. Neutral Protease NB is a protease from C. histolyticum. It has a proteolytic activity of ≥ 0.50 DMC U/mg.
For murine bone tissue, we recommend using Collagenase NB 4 Standard Grade. Collagenase NB 4 Standard Grade has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzymes | Conditions | Protocol |
|---|---|---|---|
| Human | Collagenase NB 8 | 1.5-2 PZ U/ml (NB 8), | Download Protocol |
| Neutral Protease NB | 0.25 DMC U/ml (NP), | ||
| 2-4 h , 37 °C |
Isolation of Schwann Cells from Neural Tissue with Collagenase NB
We recommend using Collagenase NB 4 Standard Grade for isolation of Schwann cells from rodent neural tissue. Collagenase NB 4 Standard Grade has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.
For clinical applications with human neural tissue, we recommend using Collagenase NB 1 GMP Grade with Neutral Protease NB GMP Grade. Collagenase NB 1 GMP Grade is a highly purified collagenase with a high collagenolytic activity (≥ 3.0 PZ U/mg) and very low proteolytic side activities. Research quality Collagenase NB 1 Premium Grade is also available. Neutral Protease NB GMP Grade is a protease from C. histolyticum. It has a proteolytic activity of ≥ 0.50 DMC U/mg. Research grade Neutral Protease NB is also available.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzyme | Conditions | Protocol |
|---|---|---|---|
| Human | Collagenase NB 1 | 0.5 PZ U/ml, 2 DCM U/ml, | Download Protocol |
| Neutral Protease NB GMP Grade | 18 h, 37 °C | ||
| Rodent | Collagenase NB 4 | 0.2-0.3 PZ U/ml, | Download Protocol |
| 1-3 h, 37 °C |
Isolation of Stem Cells from Wharton’s Jelly with Collagenase NB
We recommend using Collagenase NB 4 Standard Grade for isolation of human stem cells from Wharton’s jelly. Collagenase NB 4 Standard Grade has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Research Application | Enzyme | Conditions |
|---|---|---|---|
| Human | Basic | Collagenase NB 4 | 0.13-0.15 PZ U/ml, |
| 1-6 h , 37 °C | |||
| Human | Clinical | Collagenase NB 6 | 0.13-0.15 PZ U/ml, |
| 1-6 h , 37 °C |
Isolation of Adipose-Derived Stem Cells with Collagenase NB
We recommend using Collagenase NB 4 Standard Grade for isolation of adipose-derived stem cells (ADSCs) from human and mouse tissue. Collagenase NB 4 Standard Grade has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.
For clinical applications, we recommend either using Collagenase NB 6 GMP Grade or Collagenase AF-1 with Neutral Protease AF. Collagenase NB 6 has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Research Application | Enzyme | Conditions | Protocol |
|---|---|---|---|---|
| Human | Basic | Collagenase NB 4 | 0.2-0.3 PZ U/ml*, | Download Protocol |
| 30-60 min, 37 °C | ||||
| Murine | Basic | Collagenase NB 4 | 0.2-0.3 PZ U/ml**, | Download Protocol |
| 30-60 min, 37 °C | ||||
| Human | Clinical | Collagenase NB 6 | 0.2-0.3 PZ U/ml*, | Download Protocol |
| 30-60 min, 37 °C | ||||
| Human | Clinical | Collagenase AF-1 | 0.4 PZ U/ml++ & 0.2 DMC U/ml++, | Download Protocol |
| Neutral Protease AF | 50 min, 37 °C |
Isolation of Tumor Cells from Colon Tissue with Collagenase NB
We recommend using Collagenase NB 4G Proved Grade for isolation of tumor cells from human colon tissue. Collagenase NB 4G Proved Grade has a collagenase activity of ≥ 0.18 PZ U/mg and a balanced mix of proteolytic side activities.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzyme | Conditions | Protocol |
|---|---|---|---|
| Human | Collagenase NB 4G | 0.5-1 PZ U/ml, | Download Protocol |
| RT |
Isolation of Tumor Cells from Human Breast, Uterus, Thyroid Gland or Lung Tissue with Collagenase NB
We recommend using Collagenase NB 8 Broad Range for isolation of tumor cells from human breast, uterus, thyroid gland, and lung tissue. Collagenase NB 8 Broad Range is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzyme | Conditions | Protocol |
|---|---|---|---|
| Human | Collagenase NB 8 | 3.5-5.5 PZ U/ml, | Download Protocol |
| 3-5 h, 37 °C |
Isolation of Tumor Cells from Murine Lung Tissue with Collagenase NB
We recommend using Collagenase NB 7D Gentle Grade for isolation of tumor cells from murine lung tissue. Collagenase NB 7D Gentle Grade is characterized by slightly higher collagenase activity with low tryptic activities ideal to preserve cell-surface proteins.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzyme | Conditions |
|---|---|---|
| Murine | Collagenase NB 7D | 0.5-0.6 PZ U/ml, |
| 1.5-2 h, 37 °C, | ||
| add 100 μg/mL DNase |
Isolation of Tumor Cells from Murine Skin with Collagenase NB
We recommend using Collagenase NB 8 Broad Range for isolation of tumor cells from murine skin. Collagenase NB 8 Broad Range characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzyme | Conditions |
|---|---|---|
| Mouse | Collagenase NB 8 | 0.12 PZ U/ml, |
| 30 min, 37 °C |
By Tissue Type
Isolation of Adipocytes/Adipose-Derived Stem Cells with Collagenase NB
We recommend using Collagenase NB 4 Standard Grade for isolation of adipocytes/adipose-derived stem cells (ADSCs) from human and mouse tissue. Collagenase NB 4 Standard Grade has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.
For clinical applications, we recommend either using Collagenase NB 6 GMP Grade or Collagenase AF-1 with Neutral Protease AF. Collagenase NB 6 has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Research Application | Enzyme | Conditions | Protocol |
|---|---|---|---|---|
| Human | Basic | Collagenase NB 4 | 0.2-0.3 PZ U/ml*, | Download Protocol |
| 30-60 min, 37 °C | ||||
| Murine | Basic | Collagenase NB 4 | 0.2-0.3 PZ U/ml**, | Download Protocol |
| 30-60 min, 37 °C | ||||
| Human | Clinical | Collagenase NB 6 | 0.2-0.3 PZ U/ml*, | Download Protocol |
| 30-60 min, 37 °C | ||||
| Human | Clinical | Collagenase AF-1 | 0.4 PZ U/ml++ & 0.2 DMC U/ml++, | Download Protocol |
| Neutral Protease AF | 50 min, 37 °C |
Isolation of Osteoblasts with Collagenase NB
We recommend using Collagenase NB 8 Broad Range with Neutral Protease NB for isolation of osteoblasts from human tissue. Collagenase NB 8 Broad Range is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities. Neutral Protease NB is a protease from C. histolyticum. It has a proteolytic activity of ≥ 0.50 DMC U/mg.
For murine bone tissue, we recommend using Collagenase NB 4 Standard Grade. Collagenase NB 4 Standard Grade has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzymes | Conditions | Protocol |
|---|---|---|---|
| Human | Collagenase NB 8 | 1.5-2 PZ U/ml (NB 8), | Download Protocol |
| Neutral Protease NB | 0.25 DMC U/ml (NP), | ||
| 2-4 h , 37 °C |
Isolation of Chondrocytes with Collagenase NB
We recommend using Collagenase NB 4 Standard Grade for isolation of chondrocytes from murine, bovine and human cartilage. Collagenase NB 4 Standard Grade has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.
For clinical applications, we recommend using Collagenase NB 6 GMP Grade has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Research Application | Enzyme | Conditions | Protocol |
|---|---|---|---|---|
| Bovine | Basic | Collagenase NB 4 | 0.2-0.3 PZ U/ml, | Download Protocol |
| 14-16 h, 37 °C | ||||
| Human | Basic | Collagenase NB 4 | 0.3-0.4 PZ U/ml, | Download Protocol |
| 4-16 h, 37 °C | ||||
| Mouse (newborn) | Basic | Neutral Protease NB | Consecutive digestion steps: | Download Protocol |
| 0.5-1 DMC U/ml (NP), | ||||
| 15-20 min, 37 °C | ||||
| Collagenase NB 4 | 0.3 PZ U/ml (NB 4), | |||
| 30-45 min, 37 °C | ||||
| Human | Clinical | Collagenase NB 6 | 0.3-0.4 PZ U/ml, | Download Protocol |
| 4-16 h, 37 °C |
Isolation of Tumor Cells from Colon Tissue with Collagenase NB
We recommend using Collagenase NB 4G Proved Grade for isolation of tumor cells from human colon tissue. Collagenase NB 4G Proved Grade has a collagenase activity of ≥ 0.18 PZ U/mg and a balanced mix of proteolytic side activities.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzyme | Conditions | Protocol |
|---|---|---|---|
| Human | Collagenase NB 4G | 0.5-1 PZ U/ml, | Download Protocol |
| RT |
Isolation of Cells from Gastric/Intestine Tissue with Collagenase NB
We recommend using Collagenase NB 4G Proved Grade for isolation of epithelial cells from gastric mucosa and mastocytes from human intestinal mucosa. Collagenase NB 4G Proved Grade has a collagenase activity of ≥ 0.18 PZ U/mg and a balanced mix of proteolytic side activities.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzyme | Conditions | Protocol |
|---|---|---|---|
| Human | Collagenase NB 4G | 0.5 PZ U/ml, | Download Protocol |
| 1 h, 37 °C |
Isolation of Cardiomyocytes from Cardiac Muscle Tissue
We recommend using Collagenase NB 8 Broad Range for isolation of cardiomyocytes from human and rat cardiac muscle tissue. Collagenase NB 8 Broad Range is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzyme | Conditions | Protocol |
|---|---|---|---|
| Rat (adult) | Collagenase NB 8 | 0.5 PZ U/ml, | Download Protocol |
| 45-60 min, 37 °C | |||
| Human* | Collagenase NB 8 | 0.8-1.0 PZ U/ml, | Download Protocol |
| 2-3.5 h, 37 °C |
Isolation of Endothelial Cells from Aorta Tissue
We recommend using Collagenase NB 4 Standard Grade for isolation of endothelial cells from rodent aortas. Collagenase NB 4 Standard Grade has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzymes | Conditions | Protocol |
|---|---|---|---|
| Rodent | Collagenase NB 4 | 0.12-0.15 PZ U/ml, | Download Protocol |
| 10-20 min, 37 °C |
Isolation of Hepatocytes from Liver Tissue with Collagenase NB
We recommend using Collagenase NB 4G Proved Grade for isolation of hepatocytes from human, mouse and rat liver tissue. Collagenase NB 4G Proved Grade has a collagenase activity of ≥ 0.18 PZ U/mg and a balanced mix of proteolytic side activities.
For pig liver tissue, we recommend using Collagenase NB 8 Broad Range. Collagenase NB 8 Broad Range characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.
For clinical applications, we recommend using Collagenase NB 6 GMP Grade. Collagenase NB 6 GMP Grade has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Research Application | Enzymes | Conditions | Protocol |
|---|---|---|---|---|
| Human | Basic | Collagenase NB 4G | 0.25-0.35 PZ U/ml, | Download Protocol |
| 10-20 min, 37 °C | ||||
| Mouse | Basic | Collagenase NB 4G | 0.08-0.11 PZ U/ml, | Download Protocol |
| 5-15min, 37 °C | ||||
| Pig | Basic | Collagenase NB 8 | 0.2-0.3 PZ U/ml | Download Protocol |
| Rat (adult) | Basic | Collagenase NB 4G | 0.12-0.15 PZ U/ml, | Download Protocol |
| 10-25 min, 37 °C | ||||
| Human | Clinical | Collagenase NB 6 | 0.25-0.35 PZ U/ml, | Download Protocol |
| 10-20 min, 37 °C |
Isolation of Lung Cells with Collagenase NB
We recommend using Collagenase NB 8 Broad Range for isolation of lung tumor cells from human tissue. Collagenase NB 8 Broad Range characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.
For murine lung tissue, we recommend using Collagenase NB 4 Standard Grade. Collagenase NB 4 Standard Grade has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzyme | Conditions | Protocol |
|---|---|---|---|
| Mouse | Collagenase NB 4 | 0.2 PZ U/ml, | Download Protocol |
| 1 h, 37 °C | |||
| Human | Collagenase NB 8 | 3.5-5.5 PZ U/ml, | |
| 3-5 h, 37 °C |
Isolation of Myocytes from Skeletal Muscle Tissue with Collagenase NB
We recommend using Collagenase NB 8 Broad Range for isolation of myocytes from human skeletal muscle. Collagenase NB 8 Broad Range characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzyme | Conditions | Protocol |
|---|---|---|---|
| Human | Collagenase NB 8 | 0.4-0.6 PZ U/ml, | Download Protocol |
| 37 °C |
Isolation of Cardiomyocytes from Cardiac Muscle Tissue
We recommend using Collagenase NB 8 Broad Range for isolation of cardiomyocytes from human and rat cardiac muscle tissue. Collagenase NB 8 Broad Range is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzyme | Conditions | Protocol |
|---|---|---|---|
| Rat (adult) | Collagenase NB 8 | 0.5 PZ U/ml, | Download Protocol |
| 45-60 min, 37 °C | |||
| Human* | Collagenase NB 8 | 0.8-1.0 PZ U/ml, | Download Protocol |
| 2-3.5 h, 37 °C |
Isolation of Neurons from Dorsal Root Ganglia with Collagenase NB
We recommend using Collagenase NB 4 Standard Grade or Collagenase NB 6 GMP Grade for isolation of neurons from human dorsal root ganglia. Collagenase NB 4 Standard Grade has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. Collagenase NB 6 GMP Grade has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.
For rodent dorsal root ganglia, we recommend using Collagenase NB 4 Standard Grade.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzymes | Conditions | Protocol |
|---|---|---|---|
| Human | Collagenase NB 4 | 0.15-0.25 PZ U/ml, | Download Protocol |
| or | 30-60 min, 37 °C | ||
| Collagenase NB 6 | |||
| Rodent | Collagenase NB 4 | 0.15-0.25 PZ U/ml, | Download Protocol |
| 30-60 h, 37 °C** |
Isolation of Schwann Cells from Neural Tissue with Collagenase NB
We recommend using Collagenase NB 4 Standard Grade for isolation of Schwann cells from rodent neural tissue. Collagenase NB 4 Standard Grade has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.
For clinical applications with human neural tissue, we recommend using Collagenase NB 1 GMP Grade with Neutral Protease NB GMP Grade. Collagenase NB 1 GMP Grade is a highly purified collagenase with a high collagenolytic activity (≥ 3.0 PZ U/mg) and very low proteolytic side activities. Research quality Collagenase NB 1 Premium Grade is also available. Neutral Protease NB GMP Grade is a protease from C. histolyticum. It has a proteolytic activity of ≥ 0.50 DMC U/mg. Research grade Neutral Protease NB is also available.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzyme | Conditions | Protocol |
|---|---|---|---|
| Human | Collagenase NB 1 | 0.5 PZ U/ml, 2 DCM U/ml, | Download Protocol |
| Neutral Protease NB GMP Grade | 18 h, 37 °C | ||
| Rodent | Collagenase NB 4 | 0.2-0.3 PZ U/ml, | Download Protocol |
| 1-3 h, 37 °C |
Isolation of Acinar Cells from Pancreatic Tissue with Collagenase NB
We recommend using Collagenase NB 8 Broad Range for isolation of acinar cells from human pancreatic tissue. Collagenase NB 8 Broad Range is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzyme | Conditions | Protocol |
|---|---|---|---|
| Human | Collagenase NB 8 | 0.05-0.1 PZ U/ml, | Download Protocol |
| 1 h, 37 °C |
Isolation of Islets of Langerhans from Pancreatic Tissue with Collagenase NB
| Species | Research Application | Enzymes | Conditions | Protocol |
|---|---|---|---|---|
| Human | Basic | Collagenase NB 1 Premium Grade | Per gram tissue: | |
| 20 PZ U (NB 1), | ||||
| Neutral Protease NB | 0.8-1.5 DMC U (NP), | |||
| 15-30 min, 37 °C | ||||
| Porcine (adult) | Basic | Collagenase NB 8 | Per gram tissue: | Download Protocol |
| 4.4 PZ U (NB 8), | ||||
| Neutral Protease NB | 0.5-0.7 DMC U (NP), | |||
| 37 °C | ||||
| Porcine (neonatal) | Basic | Collagenase NB 4 | 0.17-0.2 PZ U/ml, | Download Protocol |
| 10-20 min, 37 °C | ||||
| Rodent | Basic | Collagenase NB 8 | 1.5-1.7 PZ U/ml | Download Protocol |
| 14-17 min, 37 °C | ||||
| Human | Clinical | Collagenase NB 1 GMP Grade | Per gram tissue: | |
| 20 PZ U (NB 1), | ||||
| Neutral Protease NB GMP Grade | 0.8-1.5 DMC U (NP), | |||
| 15-30 min, 37 °C | ||||
| Porcine (adult) | Clinical | Collagenase NB 1 | Per gram tissue: | |
| 15-18 PZ U (NB 1), | ||||
| Neutral Protease NB GMP Grade | 0.8-1.3 DMC U (NP), | |||
| 15-30 min, 37 °C | ||||
| Porcine (neonatal) | Clinical | Collagenase NB 6 | 0.17-0.2 PZ U/ml, | |
| 10-20 min, 37 °C |
Isolation of Oocytes from Xenopus laevis with Collagenase NB
We recommend using Collagenase NB 4G for isolation of oocytes from Xenopus laevis. Collagenase NB 4G Proved Grade has a collagenase activity of ≥ 0.18 PZ U/mg and a balanced mix of proteolytic side activities.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzyme | Conditions | Protocol |
|---|---|---|---|
| Frog | Collagenase NB 4G | 0.3-0.5 PZ U/ml, | Download Protocol |
| 1.5-2 h, RT |
Isolation of Amnion and Chorion Cells from Human Fetal Tissue with Collagenase NB
We recommend using Collagenase NB 8 Broad Range for isolation of amnion and chorion cells from human fetal tissue. Collagenase NB 8 Broad Range is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzyme | Conditions | Protocol |
|---|---|---|---|
| Human | Collagenase NB 8 | 3.5-5.5 PZ U/ml, | Download Protocol |
| 3-5 h, 37 °C |
Isolation of Endometrial Cells from Human Cervical Tissue with Collagenase NB
We recommend using Collagenase NB 4 Standard Grade or Collagenase NB 6 GMP Grade for isolation of endometrial cells from human cervical tissue. Collagenase NB 4 Standard Grade has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. Collagenase NB 6 GMP Grade has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzymes | Conditions | Protocol |
|---|---|---|---|
| Human | Collagenase NB 4 | 0.2-0.3 PZ U/ml, | Download Protocol |
| or | 30 min, 37 °C | ||
| Collagenase NB 6 |
Isolation of Endothelial Cells From Human Foreskin and Umbilical Vein with Collagenase NB
We recommend using Collagenase NB 4 Standard Grade or CCollagenase NB 6 GMP Grade for isolation of endothelial cells from human foreskin or umbilical vein. Collagenase NB 4 Standard Grade has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. Collagenase NB 6 GMP Grade has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzymes | Conditions | Protocol |
|---|---|---|---|
| Human (HDMEC) | Collagenase NB 4 | 0.5-0.7 PZ U/ml, | Download Protocol |
| or | 0.5-2 h, 37 °C | ||
| Collagenase NB 6 | |||
| Human (HUVEC) | Collagenase NB 4 | 0.15-0.25 PZ U/ml, | Download Protocol |
| or | 0.5-2 h, 37 °C | ||
| Collagenase NB 6 |
Isolation of Epithelial Cells from Murine Mammary Glands with Collagenase NB
We recommend using Collagenase NB 4 Standard Grade for isolation of epithelial cells from murine mammary gland. Collagenase NB 4 Standard Grade has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzymes | Conditions | Protocol |
|---|---|---|---|
| Mouse | Collagenase NB 4 | 0.34 PZ U/ml, | Download Protocol |
| 15-25 min, 37 °C |
Isolation of Tumor Cells from Breast and Uterus Tissue with Collagenase NB
We recommend using Collagenase NB 8 Broad Range for isolation of tumor cells from breast and uterus tissue. Collagenase NB 8 Broad Range is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzyme | Conditions | Protocol |
|---|---|---|---|
| Human | Collagenase NB 8 | 3.5-5.5 PZ U/ml, | Download Protocol |
| 3-5 h, 37 °C |
Isolation of Melanoma Cells from Murine Skin with Collagenase NB
We recommend using Collagenase NB 8 Broad Range for isolation of melanoma cells from murine skin. Collagenase NB 8 Broad Range is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzyme | Conditions |
|---|---|---|
| Mouse | Collagenase NB 8 | 0.12 PZ U/ml, |
| 30 min, 37 °C |
Isolation of Fibroblasts from Skin with Collagenase NB
We recommend using Collagenase NB 4G for isolation of fibroblasts from human and rodent skin. Collagenase NB 4G Proved Grade has a collagenase activity of ≥ 0.18 PZ U/mg and a balanced mix of proteolytic side activities.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Research Application | Enzymes | Conditions | Protocol |
|---|---|---|---|---|
| Human | Basic | Collagenase NB 4G | 0.26 PZ U/ml, | Download Protocol |
| 2 h, 37 °C | ||||
| Rodent | Basic | Collagenase NB 4G | 0.2-0.5 PZ U/ml, | Download Protocol |
| 12-20 h, 37 °C | ||||
| Human | Clinical | Collagenase NB 6 | 0.2-0.3 PZ U/ml, | Download Protocol |
| 2 h, 37 °C |
Isolation of Endothelial Cells From Human Foreskin with Collagenase NB
We recommend using Collagenase NB 4 Standard Grade or Collagenase NB 6 GMP Grade for isolation of endothelial cells from human foreskin. Collagenase NB 4 Standard Grade has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. Collagenase NB 6 GMP Grade has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzymes | Conditions | Protocol |
|---|---|---|---|
| Human | Collagenase NB 4 | 0.5-0.7 PZ U/ml, | Download Protocol |
| or | 0.5-2 h, 37 °C | ||
| Collagenase NB 6 |
Isolation of Leukocytes from Murine Skin with Collagenase NB
We recommend using Collagenase NB 7D Gentle Grade for isolation of leukocytes from murine skin. Collagenase NB 7D Gentle Grade is characterized by slightly higher collagenase activity with low tryptic activities ideal to preserve cell-surface proteins.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzyme | Conditions |
|---|---|---|
| Murine | Collagenase NB 7D | 0.18-0.22 PZ U/ml, |
| 30 min, 37 °C |
Isolation of Epithelial Cells from Thyroid Glands with Collagenase NB
We recommend using Collagenase NB 4 Standard Grade or Collagenase NB 6 GMP Grade for isolation of epithelial cells from human thyroid glands. Collagenase NB 4 Standard Grade has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. Collagenase NB 6 GMP Grade has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzymes | Conditions | Protocol |
|---|---|---|---|
| Human | Collagenase NB 4 | 0.25-0.3 PZ U/ml, | Download Protocol |
| or | RT | ||
| Collagenase NB 6 |
Isolation of Tumor Cells from Thyroid Glands with Collagenase NB
We recommend using Collagenase NB 8 Broad Range for isolation of tumor cells from human thyroid glands. Collagenase NB 8 Broad Range is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzyme | Conditions | Protocol |
|---|---|---|---|
| Human | Collagenase NB 8 | 3.5-5.5 PZ U/ml, | Download Protocol |
| 3-5 h, 37 °C |
Isolation of Tumor Cells from Colon Tissue with Collagenase NB
We recommend using Collagenase NB 4G Proved Grade for isolation of tumor cells from human colon tissue. Collagenase NB 4G Proved Grade has a collagenase activity of ≥ 0.18 PZ U/mg and a balanced mix of proteolytic side activities.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzyme | Conditions | Protocol |
|---|---|---|---|
| Human | Collagenase NB 4G | 0.5-1 PZ U/ml, | Download Protocol |
| RT |
Isolation of Tumor Cells from Human Breast, Uterus, Thyroid Gland or Lung Tissue with Collagenase NB
We recommend using Collagenase NB 8 Broad Range for isolation of tumor cells from human breast, uterus, thyroid gland, and lung tissue. Collagenase NB 8 Broad Range is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzyme | Conditions | Protocol |
|---|---|---|---|
| Human | Collagenase NB 8 | 3.5-5.5 PZ U/ml, | Download Protocol |
| 3-5 h, 37 °C |
Isolation of Tumor Cells from Murine Skin with Collagenase NB
We recommend using Collagenase NB 8 Broad Range for isolation of tumor cells from murine skin. Collagenase NB 8 Broad Range characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzyme | Conditions |
|---|---|---|
| Mouse | Collagenase NB 8 | 0.12 PZ U/ml, |
| 30 min, 37 °C |
Isolation of Tumor Cells from Murine Lung Tissue with Collagenase NB
We recommend using Collagenase NB 7D Gentle Grade for isolation of tumor cells from murine lung tissue. Collagenase NB 7D Gentle Grade is characterized by slightly higher collagenase activity with low tryptic activities ideal to preserve cell-surface proteins.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzyme | Conditions |
|---|---|---|
| Mouse | Collagenase NB 7D | 0.5-0.6 PZ U/ml, |
| 1.5-2 h, 37 °C, | ||
| add 100 μg/mL DNase |
Isolation of Stem Cells from Wharton’s Jelly with Collagenase NB
We recommend using Collagenase NB 4 Standard Grade for isolation of human stem cells from Wharton’s jelly. Collagenase NB 4 Standard Grade has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.
For clinical applications, we recommend using Collagenase NB 6 GMP Grade. Collagenase NB 6 GMP Grade has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Research Application | Enzyme | Conditions |
|---|---|---|---|
| Human | Basic | Collagenase NB 4 | 0.13-0.15 PZ U/ml, |
| 1-6 h , 37 °C | |||
| Human | Clinical | Collagenase NB 6 | 0.13-0.15 PZ U/ml, |
| 1-6 h , 37 °C |
Isolation of Endothelial Cells from Umbilical Vein with Collagenase NB
We recommend using Collagenase NB 4 Standard Grade or Collagenase NB 6 GMP Grade for isolation of endothelial cells from human umbilical vein. Collagenase NB 4 Standard Grade has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. Collagenase NB 6 GMP Grade has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.
The suggested Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
| Species | Enzymes | Conditions | Protocol |
|---|---|---|---|
| Human | Collagenase NB 4 | 0.15-0.25 PZ U/ml, | Download Protocol |
| or | 0.5-2 h, 37 °C | ||
| Collagenase NB 6 |
