HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CISD1 (RC203308, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CISD1. Positive lysates LY413042 (100ug) and LC413042 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-CISD1 monoclonal antibody (1:200).
Anti-CISD1 mouse monoclonal antibody (TA500904) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CISD1 (RC203308).
Immunofluorescent staining of HeLa cells using anti-CISD1 mouse monoclonal antibody (TA500904).
Flow cytometric Analysis of Jurkat cells, using anti-CISD1 antibody (TA500904), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).