Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA500399)
HEK293T cells transfected with either RC201278 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CHEK2 antibody (TA500399), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHEK2 (RC201278, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHEK2. Positive lysates LY416128 (100ug) and LC416128 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CHEK2 monoclonal antibody.
Flow cytometric Analysis of Jurkat cells, using anti-CHEK2 antibody (TA500399), (Red), compared to a nonspecific negative control antibody, (Blue).