Immunofluorescent staining of HT29 cells using anti-CHEK2 mouse monoclonal antibody (TA500405).
HEK293T cells transfected with either RC201278 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CHEK2 antibody (TA500405), and then analyzed by flow cytometry.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CHEK2 monoclonal antibody.
Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-CHEK2 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: Colon).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHEK2 (RC201278, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHEK2. Positive lysates LY416128 (100ug) and LC416128 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (10ug) from a mouse cell line and a mouse tissue by using anti-CHEK2 monoclonal antibody (1:200).
Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and CHEK2-Knockout Hela cells (KO, Cat# LC810324) were separated by SDS-PAGE and immunoblotted with anti-CHEK2 monoclonal antibody TA500405. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control (1:500).
Anti-CHEK2 mouse monoclonal antibody (TA500405) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CHEK2 (RC201278).