HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHEK2 (RC201278, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHEK2. Positive lysates LY416128 (100ug) and LC416128 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CHEK2 monoclonal antibody.
Immunofluorescent staining of HT29 cells using anti-CHEK2 mouse monoclonal antibody (TA500390).
Flow cytometric Analysis of Jurkat cells, using anti-CHEK2 antibody (TA500390), (Red), compared to a nonspecific negative control antibody, (Blue).
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA500390)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA500390)
Immunoprecipitation (IP) of CHEK2 by using TrueMab monoclonal anti-CHEK2 antibodies (Negative control: IP without adding anti-CHEK2 antibody.). For each experiment, 500ul of DDK tagged CHEK2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-CHEK2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells transfected with either RC201278 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CHEK2 antibody (TA500390), and then analyzed by flow cytometry.