Flow cytometric Analysis of Hela cells, using anti-CHCHD5 antibody (TA502350), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHCHD5 (RC202606, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHCHD5. Positive lysates LY410209 (100ug) and LC410209 (20ug) can be purchased separately from OriGene.
HEK293T cells transfected with either RC202606 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CHCHD5 antibody (TA502350), and then analyzed by flow cytometry.