Anti-PRKG1 mouse monoclonal antibody (TA501208) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKG1 (RC220986).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKG1 (RC220986, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKG1. Positive lysates LY416766 (100ug) and LC416766 (20ug) can be purchased separately from OriGene.
HEK293T cells transfected with either RC220986 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PRKG1 antibody (TA501208), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-PRKG1 antibody (TA501208), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).