Cellufine™ ETclean

Affinity chromatography media for endotoxin removal

Cellufine™ ETclean are poly(ε-lysine) immobilized Cellufine™ cellulose spherical beads. The beads bind and remove endotoxin from your sample solution. The poly(ε-lysine) is a microbial poly(amino acid) that consists of 30-35 lysine residues produced by Streptomyces albulus. The poly(ε-lysine) acts as a ligand while the cellulose beads act as a matrix, enabling the efficient removal of endotoxin from your sample solution.

Features

Resistant against clean-up solutions including 0.2M sodium hydroxide and 2M sodium
Can remove endotoxin from a cellular product solution at physiological pH, ionic strength of µ = 0.02-1.0, and temperature range of 0-25°C
Easily reduces LPS to low concentrations compared with polymyxin-immobilized agarose gel

Name	Supplied	Wet Bead Diameter	Pore Size*
Cellufine™ ETclean-S	A slurry in 20% ethanol	ca. 40-130 µm	M_lim 2000
Cellufine™ ETclean-L	A slurry in 20% ethanol	ca. 40-130 µm	>M_lim 2×10⁶

*The pore size (molecular weight exclusion; M_lim) of the beads was estimated from calibration curves obtained by size exclusion chromatography. Pullulan and maltose were used for the M_lim determination.

Cellufine™ ETclean S

Name	Datasheet	Packsize	Order
MC Cellufine Etclean S		1 x 5 ml	View
MC Cellufine Etclean S		5 x 1 ml	View

Cellufine™ ETclean L

Name	Datasheet	Packsize	Order
MC Cellufine Etclean L		1 x 5 ml	View
MC Cellufine Etclean L		5 x 1 ml	View

Application Data

Selective adsorption of endotoxin (LPS) from a bovine serum albumin (BSA) solution by Cellufine™ ETclean beads

Cellufine™ is the trademark of JNC Corporation, Tokyo, Japan

Supporting Documents

Cellufine resin was developed by JNC and is available from AMSBIO in EU and the US

Additional Resources

Liquid Chromatography Media

Type: Brochures

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Cellufine™ ETclean

Affinity chromatography media for endotoxin removal

Features

Cellufine™ ETclean S

Cellufine™ ETclean L

Application Data

Selective adsorption of endotoxin (LPS) from a bovine serum albumin (BSA) solution by Cellufine™ ETclean beads

Supporting Documents

Additional Resources

Liquid Chromatography Media

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Cellufine™ Cellulose Chromatography Mini Columns

Cellufine™ MAX Ion Exchange Chromatography Media

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