Affinity chromatography media for endotoxin removal
Cellufine™ ETclean are poly(ε-lysine) immobilized Cellufine™ cellulose spherical beads. The beads bind and remove endotoxin from your sample solution. The poly(ε-lysine) is a microbial poly(amino acid) that consists of 30-35 lysine residues produced by Streptomyces albulus. The poly(ε-lysine) acts as a ligand while the cellulose beads act as a matrix, enabling the efficient removal of endotoxin from your sample solution.
- Resistant against clean-up solutions including 0.2M sodium hydroxide and 2M sodium
- Can remove endotoxin from a cellular product solution at physiological pH, ionic strength of µ = 0.02-1.0, and temperature range of 0-25°C
- Easily reduces LPS to low concentrations compared with polymyxin-immobilized agarose gel
|Name||Supplied||Wet Bead Diameter||Pore Size*|
|Cellufine™ ETclean-S||A slurry in 20% ethanol||ca. 40-130 µm||Mlim 2000|
|Cellufine™ ETclean-L||A slurry in 20% ethanol||ca. 40-130 µm||>Mlim 2×106|
*The pore size (molecular weight exclusion; Mlim) of the beads was estimated from calibration curves obtained by size exclusion chromatography. Pullulan and maltose were used for the Mlim determination.
Selective adsorption of endotoxin (LPS) from a bovine serum albumin (BSA) solution by Cellufine™ ETclean beads
Cellufine™ is the trademark of JNC Corporation, Tokyo, Japan
Cellufine resin was developed by JNC and is available from AMSBIO in EU and the US