Genetically engineered iPSCs for cell therapy

iPSC-Derived Cells Enable Drug Discovery & Cell Therapy Research

A range of genetically engineered iPSCs providing tailored solutions to meet your specific research needs. iPSCs provide a powerful platform for studying a wide range of cellular processes and diseases. We offer Cas9-expressing iPSCs enabling you to perform precise genetic modifications as well as reporter and knockout iPSCs for studying gene expression of interest.

Benefits

  • Improved model fidelity over cell lines and biochemical assays
  • Improved throughput and cost over animal models
  • Applications include disease modelling, personalized medicine, drug screening and toxicity testing

Cas9-Expressing iPSCs

PBMC-derived human iPS cells were engineered to express Cas9 (Streptococcus pyogenes CRISPR associated protein 9) either constitutively or upon doxycyclin induction (tet-on). These cells can be transfected or transduced with a cDNA encoding single-guide RNA(s) targeting specific gene(s) of interest to generate knockout, mutated, or knockin cells. The inducible cells allow temporal control of Cas9 expression, which limits the occurrence of off-target modifications.

NamePacksize Order
Cas9 Expressing iPS Cell Pool1 vial View
Cas9 Inducible (Tet-On) iPS Cell Pool1 vial View

StemBright™ Reporter iPSCs

StemBright™ Reporter iPSCs are engineered to express a conditional reporter gene that will respond to the activation of a transcription factor within a cell signaling pathway of interest, or to constitutively express luciferase or eGFP for cell tracking. For example, Wnt signaling is involved in the cell cycle re-entry of iPSC-derived cardiomyocytes. The TCF/LEF (Wnt) Luciferase Reporter iPS Cell pool responds to Wnt pathway activators with a quantitative, dose-dependent increase in luciferase activity. These pluripotent reporter cells can also be used to generate differentiated reporter cells.

NamePacksize Order
StemBrightâ„¢ Luciferase iPS Cell Pool1 vial View
TCF/LEF StemBrightâ„¢ Luciferase iPS Cell Pool (Wnt Pathway)1 vial View

Knockout iPSCs

Eliminating the expression of a cellular protein can provide valuable research insights. Using CRISPR/Cas9 technology, we can effectively knockout target proteins to change cellular properties as desired. For example, our B2M Knockout iPS Cell Line has the full functional capacity of iPSCs, but has very low immunogenicity, providing a useful tool for allogeneic cell therapy research.

NamePacksize Order
B2M Knockout iPS Cell Line1 vial View
iPSC colonies

Stable Cell Lines development

Stem Cell Differentiation Services

Our stem cell differentiation services offer you the chance to differentiate your human pluripotent stem cells into a range of cell types without the work! Simply send us your cells and receive differentiating or terminally differentiated cells in just 1-2 weeks.