FACSmax™ Cell Dissociation Solution
Maximize your cell dissociation efficiency
To achieve single cell suspensions with maximum viability, AMSBIO offers FACSmax™ Cell Dissociation Solution. This chemical separation solution utilizes a cocktail of enzymes carefully calibrated to break up clumped cells. At the same time, FACSmax™ Solution leaves the plasma membrane and intracellular components intact, allowing for maximum cell viability.
In comparative studies with standard mechanical and chemical dissociation methods, FACSmax™ Cell Dissociation Solution has proven its superior effectiveness and ability to generate healthy single cell suspensions that are optimal for accurate cell counting, flow cytometry, viral transfection assays, cell sorting, and bioreactor scale-up.
Features
- Ready-to-use solution
- Consists of a unique combination of proteases and collagenases for optimal performance
- Results in homogeneous single cell suspension
Benefits
- Dissociates clumped cells in minutes
- Saves time - no need for extra PBS washing steps
- Yields accurate, reproducible cell counts
- Maximum cell viability is achieved through gentle cell disaggregation
Real World Application
Read about how Tim Czopka, Laura Hoodless and colleagues at University of Edinburgh using FACSmax™ for single-cell sorting of oligodendrocytes and oligodendrocyte precursor cells (OPCs).
Dissociation of MCF-7 Cells Using FACSmax™ Solution
MCF-7 cells were harvested at 90% confluency from a T150 culture flask using a cell scraper, centrifuged and resuspended in 10 ml of complete medium.
Untreated MCF-7 cells
Fig. 1. 0.1 ml of suspended cells were transferred to an Eppendorf tube, to which 0.10 ml of PBS was added. The cells were vortexed and incubated at room temperature for 5 minutes then mounted on a glass slide and photographed. MCF-7 cells are adherent and grow in clumps. They are very difficult to dissociate.
FACSmax™ treated MCF-7 cells
Fig. 2. 0.1 ml of suspended cells were transferred to an Eppendorg tube, to which 0.10 ml of cold FACSmax™ cell dissociation solution was added. The cells were vortexed and incubated at room temperature for 5 minutes then mounted on a glass slide and photographed. After treatment with FACSmax™ a single cell suspension makes for easy and accurate cell counting.
Citations
Fluorescence-activated cell sorting (FACS) of fluorescently tagged cells from zebrafish larvae for RNA isolation
Martha Manoli & Wolfgang Driever (2012) Cold Spring Harb Protoc.
Marsupial single-cell transcriptomics provides an atlas of developmental heterochrony
Menchero, S., Barrington, C., Alanis-Lobato, G., Wazeer Varsally, Niakan, K.K. and James (2024). bioRxiv (Cold Spring Harbor Laboratory).
