Western blot analysis of extracts (35ug) from 5 different cell lines by using anti-CDX2 monoclonal antibody (1:500).
Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and CDX2-Knockout 293T cells (KO, Cat# LC810043) were separated by SDS-PAGE and immunoblotted with anti-CDX2 monoclonal antibody TA500251. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control (1:500).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDX2 (RC204883, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDX2. Positive lysates LY420044 (100ug) and LC420044 (20ug) can be purchased separately from OriGene.
Immunofluorescent staining of 293T cells using anti-CDX2 mouse monoclonal antibody (TA500251, green, upper left; merged, upper right) or Isotype control ( merged, lower right). Cell nuclei were stained with DAPI (blue, lower left) (1:100).