HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN3 (RC213080, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDKN3. Positive lysates LY417450 (100ug) and LC417450 (20ug) can be purchased separately from OriGene.
Anti-CDKN3 mouse monoclonal antibody (TA503593) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CDKN3 (RC213080).
HEK293T cells transfected with either RC213080 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CDKN3 antibody (TA503593), and then analyzed by flow cytometry.