Anti-CDKN3 mouse monoclonal antibody (TA503582) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CDKN3 (RC213080).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN3 (RC213080, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDKN3. Positive lysates LY417450 (100ug) and LC417450 (20ug) can be purchased separately from OriGene.
HEK293T cells transfected with either RC213080 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CDKN3 antibody (TA503582), and then analyzed by flow cytometry.