Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and CDK2-Knockout 293T cells (KO, Cat# LC810794) were separated by SDS-PAGE and immunoblotted with anti-CDK2 monoclonal antibody TA503104, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.
Flow cytometric Analysis of Hela cells, using anti-CDK2 antibody, (Red), compared to PBS (green) and a nonspecific negative control antibody, (Blue) (1:100).
Anti-CDK2 mouse monoclonal antibody (TA503104) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CDK2 (RC200494).
HEK293T cells transfected with either RC200494 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CDK2 antibody (TA503104) or isotype control antibody (green), and then analyzed by flow cytometry (1:100).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDK2 (RC200494, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDK2. Positive lysates LY419741 (100ug) and LC419741 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug) from 14 cell lines by using anti-CDK2 monoclonal antibody (1:500).