HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDK2 (RC200494, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDK2. Positive lysates LY419741 (100ug) and LC419741 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CDK2 monoclonal antibody.
Anti-CDK2 mouse monoclonal antibody (TA502833) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CDK2 (RC200494).
Flow cytometric Analysis of Jurkat cells, using anti-CDK2 antibody (TA502833), (Red), compared to a nonspecific negative control antibody, (Blue).
Flow cytometric Analysis of Hela cells, using anti-CDK2 antibody (TA502833), (Red), compared to a nonspecific negative control antibody, (Blue).
HEK293T cells transfected with either RC200494 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CDK2 antibody (TA502833), and then analyzed by flow cytometry.