CD4+ T cells/ Negatively Selected (Human)

Product Code
79752
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Cryopreserved vial (10 x 10^6 cells) of CD4+ T cells that were negatively selected from freshly isolated primary human peripheral blood mononuclear cells (PBMCs). The PBMCs came from a healthy donor/ and were isolated from whole blood or leukapheresis samples using a Ficoll gradient. Magnetic antibodies to monocytes/ granulocytes/ CD8+ T cells/ gamma/delta T cells and other immune subsets present in PBMCs were then used to purify untouched CD4+ T cells via immunomagnetic separation. Before and after CD4+ T cell isolation/ the cells were stained to evaluate purity and viability by flow cytometry. Cells were cryopreserved in CryoStor CS10 cryopreservation medium (Stemcell/ #07930) at a controlled rate.Source
Normal human PBMC from Leukapheresis Sample
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SKU 79752
Size 10 million cells
Alternate SKU AMS.79752
Species Human
Gene synonyms T-Cell Surface Glycoprotein CD4, T-Cell Surface Antigen T4/Leu-3, CD4 Molecule
Storage Store cells at -135C or colder. Thawed cells should be used immediately for downstream applications. Because these are primary cells/ we do not recommend maintaining these cells in culture for long periods of time.
Shipping Temp -80C (dry ice)
Supplier Name BPS