Flow cytometric Analysis of living RBC-lysed human peripheral blood cells, using anti-CD4 antibody (TA500483), (Red), compared to an IgG isotype control, (green), and negative control (PBS), (Blue) (1:100).
Flow cytometric Analysis of live Jurkat cells, using anti-CD4 antibody (TA500483 at 1:100, Red), compared to IgG1 isotype control (Black).
Flow cytometric Analysis of living Molt-4 cells, using anti-CD4 antibody (TA500483) (Red), compared PBS (Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4. Positive lysates LY400209 (100ug) and LC400209 (20ug) can be purchased separately from OriGene.