Immunoprecipitation (IP) of FCGR2A by using TrueMab monoclonal anti-FCGR2A antibodies (Negative control: IP without adding anti-FCGR2A antibody.). For each experiment, 500ul of DDK tagged FCGR2A overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-FCGR2A antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCGR2A (RC205786, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCGR2A. Positive lysates LY402871 (100ug) and LC402871 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (10ug) from 1 cell line by using anti-FCGR2A monoclonal antibody at 1:200.
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-FCGR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120Â°C for 3min, TA500653) (1:500)
HEK293T cells transfected with either RC205786 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FCGR2A antibody (TA500653), and then analyzed by flow cytometry.