Anti-BSG mouse monoclonal antibody (TA501188) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BSG (RC219464).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BSG (RC219464, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BSG. Positive lysates LY419777 (100ug) and LC419777 (20ug) can be purchased separately from OriGene.
HEK293T cells transfected with either RC219464 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BSG antibody (TA501188), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-BSG antibody (TA501188), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).