Cas9 Inducible (Tet-On) iPS Cell Pool

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AMS.78845
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The Cas9 Inducible (Tet-On) iPS Cell Pool expresses the Cas9 (Streptococcus pyogenes CRISPR associated protein 9) gene under the control of a tight TRE tetracycline-inducible promoter, introduced into PBMC-derived human iPS cells via lentiviral transduction [Cas9 Lentivirus (inducible TET on), AMSBIO #78794]. Cas9 expression can be induced with doxycycline treatment, allowing temporal control of its expression. Cas9 Inducible (Tet-On) iPS cells can be transduced with single-guide RNA (sgRNA) targeting gene(s) of interest to generate genetically engineered cell pools or cell lines.
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SKU AMS.78845
Size 1 vial
Application • Study molecular pathways using temporally controlled induction of gene editing.,• Generation of sgRNA-edited iPS cell pools.,• Screening of pooled sgRNAs libraries to identify pluripotency, differentiation, and drug toxicity genetic modulators.,
Gene synonyms iPSC
Storage Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.As this is a cell pool and not a cell line, AMSBIO cannot guarantee the stability of the
Shipping Temp -80°C (dry ice)
Supplier Name BPS Bioscience Inc
For more details visit our Genetically engineered iPSCs page >