Immunofluorescent staining of 293T cells transfected by pCMV6-ENTRY CALCOCO2 (RC203843) using anti-CALCOCO2 antibody (TA502106/green, upper left; DAPI/blue, lower left; MERGED, upper right). 293T cells transfected with empty vector served as a negative control (MERGED, lower right) (1:100).
HEK293T cells transfected with either RC203843 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CALCOCO2 antibody (TA502106), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CALCOCO2 (RC203843, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CALCOCO2 (1:2000).