Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-C9orf41 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA501300, Dilution 1:50)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C9orf41 (RC205116, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C9or
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-C9orf41 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).
Anti-C9orf41 mouse monoclonal antibody (TA501300) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY C9orf41 (RC205116).
HEK293T cells transfected with either RC205116 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-C9orf41 antibody (TA501300), and then analyzed by flow cytometry.