HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C2orf40 (RC206239, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C2orf40. Positive lysates LY403168 (100ug) and LC403168 (20ug) can be purchased separately from OriGene.
HEK293T cells transfected with either RC206239 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-C2orf40 antibody (TA502718), and then analyzed by flow cytometry.
Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-C2orf40 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).
Flow cytometric Analysis of Hela cells, using anti-C2orf40 antibody (TA502718), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).