HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C1orf50 (RC200134, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C1orf50. Positive lysates LY411368 (100ug) and LC411368 (20ug) can be purchased separately from OriGene.
Anti-C1orf50 mouse monoclonal antibody (TA502902) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY C1orf50 (RC200134 ).
HEK293T cells transfected with either RC200134 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-C1orf50 antibody (TA502902), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-C1orf50 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA502902)