HEK293T cells transfected with either RC200134 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-C1orf50 antibody (TA502903), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-C1orf50 antibody (TA502903), (Red), compared to a nonspecific negative control antibody, (Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C1orf50 (RC200134, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C1orf50. Positive lysates LY411368 (100ug) and LC411368 (20ug) can be purchased separately from OriGene.
Anti-C1orf50 mouse monoclonal antibody (TA502903) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY C1orf50 (RC200134 ).