HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C17orf28 (RC206740, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C17orf28. Positive lysates LY403066 (100ug) and LC403066 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-C17orf28 monoclonal antibody (1:200).
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-C17orf28 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA501369, Dilution 1:50)