Western blot analysis of extracts (35ug) from 2 different tissues and Jurkat cells by using anti-BTK monoclonal antibody (1:500).
Anti-BTK mouse monoclonal antibody (TA501102) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTK (RC211582).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTK (RC211582, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTK. Positive lysates LY424947 (100ug) and LC424947 (20ug) can be purchased separately from OriGene.
HEK293T cells transfected with either pCMV6-ENTRY BTK (RC211582) (Red) or empty vector control plasmid (Blue) were immunostained with anti-BTK mouse monoclonal (TA501102), and then analyzed by flow cytometry.