HEK293T cells transfected with either RC212185 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-VWA5A antibody (TA501637), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VWA5A (RC212185, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VWA5A. Positive lysates LY402354 (100ug) and LC402354 (20ug) can be purchased separately from OriGene.
Anti-VWA5A mouse monoclonal antibody (TA501637) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY VWA5A (RC212185).
Flow cytometric Analysis of Jurkat cells, using anti-VWA5A antibody (TA501637), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).