HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VWA5A (RC212185, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VWA5A. Positive lysates LY402354 (100ug) and LC402354 (20ug) can be purchased separately from OriGene.
HEK293T cells transfected with either RC212185 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-VWA5A antibody (TA501644), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-VWA5A antibody (TA501644), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).