Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ARNTL monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).
Figure from citation: Immunohistochemistry of ARNTL (BMAL1) protein level by using anti-ARNTL antibody in coronal sections of the main olfactory bulb (MOB) portion of 7-day-old rabbits. Dilution: 1:200 View Citation
Flow cytometric Analysis of Jurkat cells, using anti-ARNTL antibody (TA501229), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARNTL (RC207870, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARNTL. Positive lysates LY400474 (100ug) and LC400474 (20ug) can be purchased separately from OriGene.
Anti-ARNTL mouse monoclonal antibody (TA501229) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ARNTL (RC207870).
HEK293T cells transfected with either RC207870 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ARNTL antibody (TA501229), and then analyzed by flow cytometry.