Mimetix® scaffolds mimic an extracellular matrix by providing an ideal architectural environment to support the growth of cells in 3D. They are created by electrospinning medical-grade polymer poly(L-lactide) (PLLA) into microfibres, which are highly consistent with regard to fibre diameter and pore size, resulting in excellent reproducibility of cell-based assays. Mimetix® aligned microfiber scaffolds provide a physical structure for the 3D culture of cells from tissues such as the central nervous system, skeletal muscle and heart where orientation influences cell growth and behavior. The Mimetix® scaffold is incorporated into standard SBS footprint well plate frames (12- and 96-well) with bases of superior optical clarity and minimal base distortion. The aligned scaffolds are thin enough to allow microscopic imaging.