Western blot analysis of extracts (10ug) from 8 Human tissue by using anti-BIRC5 monoclonal antibody at 1:200 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-BIRC5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120Â°C for 3min, TA501245, Dilution 1:50)
HEK293T cells transfected with either RC205935 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BIRC5 antibody (TA501245), and then analyzed by flow cytometry.
Immunofluorescent staining of 293T cells transfected by pCMV6-ENTRY BIRC5 (RC205935) using anti-BIRC5 antibody (TA501245/green, upper left; DAPI/blue, lower left; MERGED, upper right). 293T cells transfected with empty vector served as a negative control (MERGED, lower right) (1:100).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BIRC5 (RC205935, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BIRC5. Positive lysates LY400469 (100ug) and LC400469 (20ug) can be purchased separately from OriGene.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-BIRC5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120Â°C for 3min, TA501245, Dilution 1:50)