Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CTNNB1 monoclonal antibody.
Anti-CTNNB1 mouse monoclonal antibody (TA502301) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CTNNB1 (RC208947).
HEK293T cells transfected with either RC208947 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CTNNB1 antibody (TA502301), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CTNNB1 (RC208947, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTNNB1. Positive lysates LY419662 (100ug) and LC419662 (20ug) can be purchased separately from OriGene.