HEK293T cells transfected with either RC218436 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BEST3 antibody (TA502154), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BEST3 (RC218436, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BEST3. Positive lysates LY407540 (100ug) and LC407540 (20ug) can be purchased separately from OriGene.
Anti-BEST3 mouse monoclonal antibody (TA502154) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BEST3 (RC218436).
Flow cytometric Analysis of Jurkat cells, using anti-BEST3 antibody (TA502154), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).