Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-BECN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA502616)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BECN1 monoclonal antibody.
Anti-BECN1 mouse monoclonal antibody (TA502616) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BECN1 (RC201629).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BECN1 (RC201629, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BECN1.
HEK293T cells transfected with either RC201629 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BECN1 antibody (TA502616), and then analyzed by flow cytometry.